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KMID : 0525720130180040243
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Journal of Chitin and Chitosan
2013 Volume.18 No. 4 p.243 ~ p.249
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Gene Delivery using Folate and Poly-L-lysine grafted O-carboxymethyl Chitosan Copolymer in Tumor Cell
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Jung Gyeong-Won
Park Sun-Jung
Kim Young-Min
Nam Joung-Pyo
Choi Chang-Yong
Park Seong-Cheol
Nah Jae-Woon
Jang Mi-Kyeong
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Abstract
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In present study, PLL-OCMCh-PEG-FA (POCPF) was prepared to apply the cell targeting gene delivery carrier. The POCPF copolymer was characterized by 1-proton nuclear magnetic resonance ( 1 H-NMR). The result indicated that POCPF has been successfully synthesized. The particle size of the POCPF was 188 ~ 320nm, which was measured by DLS (Dynamic Light Scattering). In addition, gel retardation assay showed that the POCPF has potential to bind with pDNA. Also, it showed that POCPF can be protected pDNA from DNase. The gene transfection efficiency of the POCPF was evaluated in HCT119 cells. The result showed that transfection efficiency of the POCPF was better than the OCMCh and PLL (Poly-L-lysine) via receptor mediate endocytosis between FA and FA receptor on cancer cell surface. The results demonstrated that POCPF copolymer is good candidate as a gene carrier for gene therapy.
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KEYWORD
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O-carboxymethyl chitosan, Poly-L-lysine, Folate, Gene
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